October 2016

Research articles

1. 1. Cell Biology
   2. Developmental Biology

   Analysis of cellular behavior and cytoskeletal dynamics reveal a constriction mechanism driving optic cup morphogenesis

   María Nicolás-Pérez, Franz Kuchling ... Juan R Martínez-Morales

   High-resolution live imaging analysis shows a constriction mechanism that drives zebrafish optic cup morphogenesis and highlights the role of the extracellular matrix in transmitting tensions beyond the cellular level.
2. 1. Cell Biology
   2. Developmental Biology

   bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring

   Vítor Trovisco, Katsiaryna Belaya ... Daniel St Johnston

   The localisation of bicoid mRNA depends on its microtubule-independent anchoring at the oocyte anterior.
3. 1. Biochemistry and Chemical Biology
   2. Plant Biology

   Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis

   Satoshi Yamashita, Haruhiko Yamaguchi ... Seiji Takahashi

   The first de novo synthesis of polyisoprenoids correspond to natural rubber in vitro with the recombinant proteins is achieved by identification and cell-free translation-coupled reconstitution of the rubber biosynthetic machinery on rubber particles.
4. 1. Structural Biology and Molecular Biophysics
   2. Neuroscience

   PtdInsP₂ and PtdSer cooperate to trap synaptotagmin-1 to the plasma membrane in the presence of calcium

   Ángel Pérez-Lara, Anusa Thapa ... Reinhard Jahn

   Two acidic membrane lipids increase synaptotagmin-1 dwell time and penetration into the membrane, reducing the membrane dissociation of synaptotagmin-1.
5. 1. Neuroscience

   The influence of evidence volatility on choice, reaction time and confidence in a perceptual decision

   Ariel Zylberberg, Christopher R Fetsch, Michael N Shadlen

   A single framework of bounded evidence accumulation resolves the seemingly paradoxical effects of noise on accuracy, reaction time and confidence.
6. 1. Biochemistry and Chemical Biology

   Regulation of mTORC1 by lysosomal calcium and calmodulin

   Ruo-Jing Li, Jing Xu ... Jun O Liu

   The multipronged chemical biology approach unraveled a novel mechanism by which mTOR is regulated by the second messenger calcium.
7. 1. Neuroscience

   Dopamine neurons learn relative chosen value from probabilistic rewards

   Armin Lak, William R Stauffer, Wolfram Schultz

   When learning the reward probability of novel cues, dopamine neurons convey dissociable value and novelty signals.
8. 1. Structural Biology and Molecular Biophysics
   2. Neuroscience

   Structural determinants of adhesion by Protocadherin-19 and implications for its role in epilepsy

   Sharon R Cooper, James D Jontes, Marcos Sotomayor

   Structural and binding studies provide insight into the molecular mechanism of protocadherin-19-mediated adhesion and into the biochemical basis of neurodevelopmental disease.
9. 1. Developmental Biology
   2. Stem Cells and Regenerative Medicine

   Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

   Nidhi Sharma Dey, Parvathy Ramesh ... Lolitika Mandal

   Identification of Hematopoietic stem cells (HSCs) in the genetically amenable organism Drosophila melanogaster paves the path to address HSC biology, both in normal and pathophysiological conditions.
10. 1. Neuroscience

    Lognormal firing rate distribution reveals prominent fluctuation–driven regime in spinal motor networks

    Peter C Petersen, Rune W Berg

    Neuronal participation in generation of motor patterns in the spinal circuits is lognormal, which is an indication of a rich diversity of activity within the mean-driven as well as the fluctuation-driven regimes.
11. 1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    γ-Protocadherin structural diversity and functional implications

    Kerry Marie Goodman, Rotem Rubinstein ... Lawrence Shapiro

    Crystal structures of γ-protocadherin cell-cell recognition dimers reveal the determinants of clustered protocadherin homophilic specificity and cis interaction region structures alongside mutagenesis data identify the putative cis interface.
12. 1. Neuroscience

    Release-dependent feedback inhibition by a presynaptically localized ligand-gated anion channel

    Seika Takayanagi-Kiya, Keming Zhou, Yishi Jin

    An anion-selective ligand gated channel acts as a presynaptic auto-receptor to modulate vesicle release and neuronal excitation.
13. 1. Biochemistry and Chemical Biology
    2. Neuroscience

    Proteolytic maturation of α₂δ represents a checkpoint for activation and neuronal trafficking of latent calcium channels

    Ivan Kadurin, Laurent Ferron ... Annette C Dolphin

    Multiple roles of the auxiliary calcium channel α2δ subunits require proteolytic cleavage of the precursor protein into α2 and δ.
14. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex

    Colin Echeverría Aitken, Petra Beznosková ... Jon R Lorsch

    Eukaryotic translation initiation factor 3 (eIF3) is required to stabilize the binding of mRNA at the exit channel of the small ribosomal subunit and acts at the entry channel to accelerate mRNA recruitment to the translation preinitiation complex.
15. 1. Cell Biology

    Increasing β-catenin/Wnt3A activity levels drive mechanical strain-induced cell cycle progression through mitosis

    Blair W Benham-Pyle, Joo Yong Sim ... William James Nelson

    Mechanicals forces and local (Wnt) signaling can synergize to increase β-catenin signaling and produce unique growth phenotypes.
16. 1. Structural Biology and Molecular Biophysics

    NPAS1-ARNT and NPAS3-ARNT crystal structures implicate the bHLH-PAS family as multi-ligand binding transcription factors

    Dalei Wu, Xiaoyu Su ... Fraydoon Rastinejad

    Detailed structural analysis of NPAS1-ARNT and NPAS3-ARNT complexes, and further comparisons with other bHLH-PAS protein structures, show that this family of mammalian transcription factors have distinct ligand-binding pockets within their molecular architectures.
17. 1. Neuroscience

    Loss of MeCP2 disrupts cell autonomous and autocrine BDNF signaling in mouse glutamatergic neurons

    Charanya Sampathkumar, Yuan-Ju Wu ... Christian Rosenmund

    In mouse models of Rett syndrome, the impaired cell autonomous BDNF feed forward signaling pathway results in abnormalities in neurite outgrowth and synapse formation in excitatory neurons.
18. 1. Neuroscience

    MEF2C regulates cortical inhibitory and excitatory synapses and behaviors relevant to neurodevelopmental disorders

    Adam J Harrington, Aram Raissi ... Christopher W Cowan

    Mice that lack the autism- and schizophrenia-linked gene MEF2C in cortical neurons have an imbalance of excitatory and inhibitory synapses, and impaired social and cognitive abilities.
19. 1. Neuroscience

    Plasticity-dependent, full detonation at hippocampal mossy fiber–CA3 pyramidal neuron synapses

    Nicholas P Vyleta, Carolina Borges-Merjane, Peter Jonas

    Activity-dependent enhancement of transmitter release makes the hippocampal mossy fiber synapse a full detonator in rats.
20. 1. Chromosomes and Gene Expression
    2. Plant Biology

    Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin

    Gudrun Böhmdorfer, Shriya Sethuraman ... Andrzej T Wierzbicki

    Genome-wide identification of long non-coding RNAs produced by RNA Polymerase V identifies their role in heterochromatin maintenance on the boundaries of repressed regions.
21. 1. Neuroscience

    Distinct neural mechanisms underlie the success, precision, and vividness of episodic memory

    Franziska R Richter, Rose A Cooper ... Jon S Simons

    Combining fMRI with continuous model-based measures of retrieval enables the behavioral and neural dissociation of multiple components of episodic memory.
22. 1. Neuroscience

    Unexpected arousal modulates the influence of sensory noise on confidence

    Micah Allen, Darya Frank ... Geraint Rees

    Changes in physiological arousal – as revealed by pupil dilation and heart rate – shape our confidence in decisions about uncertain perceptual information.
23. 1. Stem Cells and Regenerative Medicine

    Live imaging reveals the progenitors and cell dynamics of limb regeneration

    Frederike Alwes, Camille Enjolras, Michalis Averof

    Long-term live imaging reveals the dynamic cell behaviors and progenitor cells that underpin the regeneration of adult limbs in the crustacean Parhyale hawaiensis.
24. 1. Epidemiology and Global Health
    2. Evolutionary Biology

    Using evolution to generate sustainable malaria control with spatial repellents

    Penelope Anne Lynch, Mike Boots

    The evolution of insecticide-avoidance behaviour can be used to generate new spatial repellents to keep malaria vectors out of homes.
25. 1. Neuroscience

    TRIM28 regulates the nuclear accumulation and toxicity of both alpha-synuclein and tau

    Maxime WC Rousseaux, Maria de Haro ... Huda Y Zoghbi

    Convergent screens targeting the levels of alpha-synuclein and tau identify TRIM28 as a driver of their stability, nuclear accumulation and subsequent toxicity.
26. 1. Neuroscience

    Internal states drive nutrient homeostasis by modulating exploration-exploitation trade-off

    Verónica María Corrales-Carvajal, Aldo A Faisal, Carlos Ribeiro

    A quantitative video tracking analysis reveals that to gain the nutrients they need, flies change their decisions to exploit foods with different nutrient contents and explore the environment according to their internal amino acid and reproductive states.
27. 1. Biochemistry and Chemical Biology

    Characterization of human translesion DNA synthesis across a UV-induced DNA lesion

    Mark Hedglin, Binod Pandey, Stephen J Benkovic

    Quantitative binding and kinetic assays on DNA polymerases η and δ define the mechanism for polymerase exchange during human translesion DNA synthesis across a UV-induced lesion on the lagging strand.
28. 1. Computational and Systems Biology

    A computational interactome and functional annotation for the human proteome

    José Ignacio Garzón, Lei Deng ... Barry Honig

    A machine-learning approach is used to predict 1.35 million interactions for 85% of the human proteome.
29. 1. Neuroscience

    Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses

    Gordon X Wang, Stephen J Smith, Philippe Mourrain

    The development of a novel, high throughput method for the sub-synaptic analysis of protein localization in molecularly classified synapse classes allows sub-compartment, multiplexed detection of proteins in synapses using wide-field microscopes.
30. 1. Microbiology and Infectious Disease

    Factors essential for L,D-transpeptidase-mediated peptidoglycan cross-linking and β-lactam resistance in Escherichia coli

    Jean-Emmanuel Hugonnet, Dominique Mengin-Lecreulx ... Michel Arthur

    Production of the L,D-transpeptidase YcbB and elevated synthesis of the (p)ppGpp alarmone reveals a new mode of peptidoglycan polymerization that bypasses the D,D-transpeptidase activity of PBPs and leads to broad-spectrum β-lactam resistance in Escherichia coli.
31. 1. Neuroscience

    Regulation of neuronal axon specification by glia-neuron gap junctions in C. elegans

    Lingfeng Meng, Albert Zhang ... Dong Yan

    Genetic study of C. elegans neural development reveals the function of glia-neuron gap junctions in neuronal axon specification, and shows that glial cells regulate neuronal intracellular pathways through gap junctions.
32. 1. Neuroscience

    Decreased motor cortex excitability mirrors own hand disembodiment during the rubber hand illusion

    Francesco della Gatta, Francesca Garbarini ... Paola Borroni

    An illusion in which individuals feel that their own hand no longer belongs to them may reflect a temporary reduction in the brain’s ability to control the movement of the hand.
33. 1. Immunology and Inflammation

    Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion

    Liang Gao, Gülce Sila Gülcüler ... Ana Martin-Villalba

    The cell surface protein CD95 mediates neutrophil slow rolling and adhesion during inflammation.
34. 1. Neuroscience

    Intramuscular Neurotrophin-3 normalizes low threshold spinal reflexes, reduces spasms and improves mobility after bilateral corticospinal tract injury in rats

    Claudia Kathe, Thomas Haynes Hutson ... Lawrence David Falcon Moon

    Delayed intramuscular gene therapy with neurotrophin-3 after corticospinal tract injury reduces spasticity and improves locomotion by treating underlying causes of spasticity.
35. 1. Neuroscience

    Midbrain dopamine neurons signal aversion in a reward-context-dependent manner

    Hideyuki Matsumoto, Ju Tian ... Mitsuko Watabe-Uchida

    Dopamine neurons signal value prediction errors (VPEs) integrating information about both reward and aversion, in low reward contexts, whereas VPEs in some dopamine neurons are distorted in high reward contexts.
36. 1. Cancer Biology
    2. Cell Biology

    TP53 exon-6 truncating mutations produce separation of function isoforms with pro-tumorigenic functions

    Nitin H Shirole, Debjani Pal ... Raffaella Sordella

    Genetic and molecular analysis of TP53 exon-6 truncating mutations reveal that these mutations, contrary to current belief, promote tumorigenesis and point towards strategies for treating cancers driven by these prevalent mutations.
37. 1. Cancer Biology
    2. Cell Biology

    Methylglyoxal, a glycolysis side-product, induces Hsp90 glycation and YAP-mediated tumor growth and metastasis

    Marie-Julie Nokin, Florence Durieux ... Akeila Bellahcène

    Carbonyl stress mediated by Methylglyoxal affects Hsp90 activity, inhibits the Hippo pathway and promotes tumor growth and metastasis in breast cancer.
38. 1. Cell Biology

    Ubiquitin-dependent folding of the Wnt signaling coreceptor LRP6

    Elsa Perrody, Laurence Abrami ... F Gisou van der Goot

    A novel folding machinery assists transmembrane proteins from the cytosolic side of the endoplasmic reticulum.
39. 1. Neuroscience

    Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding

    Naiyan Chen, Hiroki Sugihara ... Weiping Han

    Glial fibrillary acidic protein-expressing glia in the arcuate nucleus of the hypothalamus modulate feeding in mice.
40. 1. Computational and Systems Biology
    2. Neuroscience

    Continuous lateral oscillations as a core mechanism for taxis in Drosophila larvae

    Antoine Wystrach, Konstantinos Lagogiannis, Barbara Webb

    Analysis of crawling Drosophila larva and agent based simulations suggest that an intrinsic rhythm rather than distinct actions underlie taxis behaviour, providing a core mechanism on which both sensory and memory pathways can converge.
41. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    HID-1 is required for homotypic fusion of immature secretory granules during maturation

    Wen Du, Maoge Zhou ... Tao Xu

    HID-1 is a novel player mediating homotypic fusion of immature secretory granules.
42. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Control of the structural landscape and neuronal proteotoxicity of mutant Huntingtin by domains flanking the polyQ tract

    Koning Shen, Barbara Calamini ... Judith Frydman

    The polyQ tract of pathogenic Huntingtin causes aggregation when expanded in Huntington’s disease, but its two flanking domains control its conformational landscape, proteostasis and neurotoxicity.
43. 1. Developmental Biology

    Klf5 regulates muscle differentiation by directly targeting muscle-specific genes in cooperation with MyoD in mice

    Shinichiro Hayashi, Ichiro Manabe ... Yumiko Oishi

    The regulatory programs governing skeletal muscle regeneration that are controlled by Klf5 in cooperation with MyoD and Mef2 provide a potential avenue for intervention into muscle regeneration through modulation of Klf5.
44. 1. Developmental Biology
    2. Neuroscience

    Frazzled promotes growth cone attachment at the source of a Netrin gradient in the Drosophila visual system

    Orkun Akin, S Lawrence Zipursky

    Direct observation in the developing fly brain indicates that the response of a growth cone in a gradient of a guidance cue is adhesion to the source of the cue rather than attraction by the gradient.
45. 1. Developmental Biology
    2. Neuroscience

    Neuronal cell fate diversification controlled by sub-temporal action of Kruppel

    Johannes Stratmann, Hugo Gabilondo ... Stefan Thor

    A combination of temporal and sub-temporal genes combine to move the stem cell through different competence windows to generate different cell types at different time-points in Drosophila.
46. 1. Developmental Biology

    The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled

    Tripti Gupta, Arun Kumar ... Angela Giangrande

    The role of the Frazzled chemoattractant receptor is to triggers migration initiation as part of the glial developmental program induced by the Glide/Gcm transcription factor.
47. 1. Developmental Biology
    2. Neuroscience

    A cellular and regulatory map of the GABAergic nervous system of C. elegans

    Marie Gendrel, Emily G Atlas, Oliver Hobert

    GABAergic neurons in the nervous system of C. elegans have been comprehensively mapped allowing analysis of regulatory factors that program GABAergic neuron identity.
48. 1. Cancer Biology
    2. Cell Biology

    Cyclin Kinase-independent role of p21^CDKN1A in the promotion of nascent DNA elongation in unstressed cells

    Sabrina F Mansilla, Agustina P Bertolin ... Vanesa Gottifredi

    A new tumor suppressor function of p21, needed at each undamaged duplication cycle, ensures the adequate execution of the DNA replication program thereby protecting the genome integrity.
49. 1. Cell Biology

    Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction

    Junqi Huang, Ting Gang Chew ... Mohan K Balasubramanian

    Cytokinetic actomyosin ring disassembly occurs through a novel mechanism in which the increased ring curvature, generated through contraction, itself promotes the disassembly process.
50. 1. Neuroscience

    NMNAT1 inhibits axon degeneration via blockade of SARM1-mediated NAD⁺ depletion

    Yo Sasaki, Takashi Nakagawa ... Jeffrey Milbrandt

    Axonal metabolic flux analysis demonstrates that expression of NMNAT1 blocks axonal degeneration in cultured mouse neurons not by altering NAD+ synthesis, but rather by inhibiting injury-induced, SARM1-dependent NAD+ consumption.
51. 1. Neuroscience

    Primate amygdala neurons evaluate the progress of self-defined economic choice sequences

    Fabian Grabenhorst, Istvan Hernadi, Wolfram Schultz

    Neurons in the amygdala, a brain system usually associated with emotion, track progress during sequential reward-directed choices according to an internal plan in Rhesus macaques.
52. 1. Structural Biology and Molecular Biophysics

    Advances in X-ray free electron laser (XFEL) diffraction data processing applied to the crystal structure of the synaptotagmin-1 / SNARE complex

    Artem Y Lyubimov, Monarin Uervirojnangkoorn ... Axel T Brunger

    Building on previous work (Uervirojnangkoorn et al., 2015), we demonstrate how improved methods for processing XFEL diffraction data enable the determination of structures from poorly diffracting crystals.
53. 1. Neuroscience

    Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism

    Alicja Puścian, Szymon Łęski ... Ewelina Knapska

    An innovative, fully computerized approach for measuring spontaneous social behavior in mice closely follows murine ethology, eliminates crucial sources of data irreproducibility and enables fast, inexpensive assessment of sociability in group-housed subjects.
54. 1. Cell Biology
    2. Developmental Biology

    The Drosophila formin Fhos is a primary mediator of sarcomeric thin-filament array assembly

    Arkadi Shwartz, Nagaraju Dhanyasi ... Ben-Zion Shilo

    Actin is incorporated into the thin-filament arrays of skeletal muscle sarcomeres in discrete steps, and Fhos, the Drosophila homolog of FHOD-family formins, is an essential player in the process.
55. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Insights into HIV-1 proviral transcription from integrative structure and dynamics of the Tat:AFF4:P-TEFb:TAR complex

    Ursula Schulze-Gahmen, Ignacia Echeverria ... James H Hurley

    The crystal structure of the trans-activation response region (TAR) bound to HIV-1 Tat and an elongation factor, together with HDX, SHAPE, SAXS, and integrative modeling, shows how TAR binds this complex in two ways.
56. 1. Stem Cells and Regenerative Medicine

    A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids

    Briana R Dye, Priya H Dedhia ... Jason R Spence

    Building on previous work (Dye et al., 2015) showing that pluripotent stem cell derived lung organoids were immature/fetal, the current study shows that in vivo transplantation leads to mature tissue, reminiscent of adult airways.
57. 1. Developmental Biology

    Calcium handling precedes cardiac differentiation to initiate the first heartbeat

    Richard CV Tyser, Antonio MA Miranda ... Paul R Riley

    High-resolution live imaging reveals how and when the mouse heart first starts to beat during development and how the onset of beating impacts on heart muscle cell maturation and heart formation.
58. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Dynamics of mTORC1 activation in response to amino acids

    Maria Manifava, Matthew Smith ... Nicholas T Ktistakis

    Activation by amino acids causes mTORC1 to translocate transiently to the lysosomal surface.
59. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Diabetes regulates fructose absorption through thioredoxin-interacting protein

    James R Dotimas, Austin W Lee ... Richard T Lee

    Thioredoxin-Interacting Protein binds directly to fructose transporters and regulates fructose metabolism, both acutely and chronically.
60. 1. Neuroscience

    Sensory dynamics of visual hallucinations in the normal population

    Joel Pearson, Rocco Chiou ... Bard Ermentrout

    A new method for inducing, measuring and modelling visual hallucinations in healthy people reveals that hallucinations share common underlying mechanisms with normal sensory perception.
61. 1. Neuroscience

    Sexually dimorphic neuronal responses to social isolation

    Laura Senst, Dinara Baimoukhametova ... Jaideep Singh Bains

    Male and female mice react differently to social isolation but not to an acute physical stress.
62. 1. Stem Cells and Regenerative Medicine

    Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency

    Sheryl Southard, Ju-Ryoung Kim ... Christoph Lepper

    For skeletal muscle in mice, the size of the stem cell pool can be uncoupled from overall tissue size allowing for a dramatic increase in stem cell number.
63. 1. Neuroscience

    An excitatory cortical feedback loop gates retinal wave transmission in rodent thalamus

    Yasunobu Murata, Matthew T Colonnese

    The absence of functional inhibition is a developmentally transient circuit property of corticothalamic feedback that is essential for the central transmission of spontaneous retinal waves.
64. 1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Live-cell single-molecule tracking reveals co-recognition of H3K27me3 and DNA targets polycomb Cbx7-PRC1 to chromatin

    Chao Yu Zhen, Roubina Tatavosian ... Xiaojun Ren

    Live-cell single-molecule tracking reveals that hierarchical cooperation within the Polycomb Cbx7 protein between the low-affinity H3K27me3-binding module and the high-affinity DNA-binding cassette targets Polycomb repressive complex 1 to chromatin.
65. 1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    BLOS2 negatively regulates Notch signaling during neural and hematopoietic stem and progenitor cell development

    Wenwen Zhou, Qiuping He ... Wei Li

    The conserved mechanism of Notch down-regulation by endo-lysosomal trafficking plays important roles in the proliferation and differentiation of stem and progenitor cells during embryogenesis or organogenesis in vertebrates.
66. 1. Neuroscience

    Asymmetric effects of activating and inactivating cortical interneurons

    Elizabeth AK Phillips, Andrea R Hasenstaub

    The consequences of manipulating neuronal activity with techniques such as optogenetics are highly sensitive to methodological details, highlighting the need for caution when interpreting results.
67. 1. Stem Cells and Regenerative Medicine
    2. Chromosomes and Gene Expression

    Polycomb enables primitive endoderm lineage priming in embryonic stem cells

    Robert S Illingworth, Jurriaan J Hölzenspies ... Joshua M Brickman

    Polycomb enables lineage priming in mouse embryonic stem cells by establishing a barrier to commitment.
68. 1. Physics of Living Systems
    2. Cell Biology

    Cortical flow aligns actin filaments to form a furrow

    Anne-Cecile Reymann, Fabio Staniscia ... Stephan W Grill

    Compressing the actomyosin network by cortical flow causes filaments to align and form a constricting ring.
69. 1. Developmental Biology

    A widely employed germ cell marker is an ancient disordered protein with reproductive functions in diverse eukaryotes

    Michelle A Carmell, Gregoriy A Dokshin ... David C Page

    GCNA proteins comprise a previously unnoticed family of proteins that has been enriched in cells carrying a heritable genome since the invention of sexual reproduction in eukaryotes, and has had reproductive function for at least 600 million years.
70. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Ankyrin-B is a PI3P effector that promotes polarized α5β1-integrin recycling via recruiting RabGAP1L to early endosomes

    Fangfei Qu, Damaris N Lorenzo ... Vann Bennett

    Ankyrin-B – through interactions with PI3P lipids, dynactin and RabGAP1L – functions as a critical node in the protein circuitry underlying polarized recycling of α5β1-integrin to enable haptotaxis along fibronectin gradients.
71. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Atomic mutagenesis in ion channels with engineered stoichiometry

    John D Lueck, Adam L Mackey ... Christopher A Ahern

    Building on previous work (Pless, 2013), we argue that side-chain 'flip out' is a key event in potassium channel C-type inactivation, and propose a new method for encoding multiple noncanonical amino acids and controlling protein stoichiometry.
72. 1. Neuroscience

    Unique membrane properties and enhanced signal processing in human neocortical neurons

    Guy Eyal, Matthijs B Verhoog ... Idan Segev

    Models and experiments reveal that human L2/3 pyramidal neurons have distinctively low specific membrane capacitance which might have a significant impact on signal processing in human neocortex.
73. 1. Neuroscience

    An event map of memory space in the hippocampus

    Lorena Deuker, Jacob LS Bellmund ... Christian F Doeller

    Evidence suggests a common coding mechanism underlies spatial and temporal aspects of episodic memory in the human hippocampus.
74. 1. Plant Biology

    Active suppression of a leaf meristem orchestrates determinate leaf growth

    John Paul Alvarez, Chihiro Furumizu ... John L Bowman

    A meristem acting at the margins of Arabidopsis leaves is suppressed by factors acting primarily in leaves and other determinate organs.
75. 1. Structural Biology and Molecular Biophysics

    The isolated voltage sensing domain of the Shaker potassium channel forms a voltage-gated cation channel

    Juan Zhao, Rikard Blunck

    Expression of the isolated voltage sensing domain significantly alters its structural conformation as well as its gating kinetics, indicating the importance of studying the biological assembly in its entirety.
76. 1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    C. elegans GLP-1/Notch activates transcription in a probability gradient across the germline stem cell pool

    ChangHwan Lee, Erika B Sorensen ... Judith Kimble

    Analyzing the readout of Notch signaling with single RNA precision reveals that active transcription sites are the most accurate measure of Notch-dependent transcriptional activation.
77. 1. Neuroscience

    Autocorrelation structure at rest predicts value correlates of single neurons during reward-guided choice

    Sean E Cavanagh, Joni D Wallis ... Laurence T Hunt

    Variability in individual neurons' temporal receptive fields of integration is found to explain the heterogeneity of neuronal responses observed in prefrontal cortex during reward-guided decision making.
78. 1. Biochemistry and Chemical Biology
    2. Developmental Biology

    Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

    Giovanni Luchetti, Ria Sircar ... Rajat Rohatgi

    Cholesterol regulates cell-cell communication by activating the Hedgehog pathway, a central signaling system in development, regeneration and cancer.
79. 1. Chromosomes and Gene Expression

    RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes

    Nathan R Rose, Hamish W King ... Robert J Klose

    The E3 ubiquitin ligase activity of polycomb repressive complex 1 is stimulated by RYBP to support a histone modification-dependent communication between polycomb repressive complexes in mice.
80. 1. Developmental Biology
    2. Neuroscience

    A multi-protein receptor-ligand complex underlies combinatorial dendrite guidance choices in C. elegans

    Wei Zou, Ao Shen ... Kang Shen

    LECT-2 guides dendrite growth and branching via a unique ligand-receptor complex.
81. 1. Developmental Biology
    2. Plant Biology

    The Arabidopsis transcription factor ABIG1 relays ABA signaled growth inhibition and drought induced senescence

    Tie Liu, Adam D Longhurst ... M Kathryn Barton

    Arabidopsis plants slow growth and jettison leaves in response to drought via a mechanism that involves the transcription factor ABIG1.
82. 1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    A near atomic structure of the active human apoptosome

    Tat Cheung Cheng, Chuan Hong ... Christopher W Akey

    A cryo-electron microscopy model of the active human apoptosome illuminates how this "wheel of death" binds the protease procaspase-9 to initiate programmed cell death.
83. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble

    Matti Turtola, Georgiy A Belogurov

    NusG enhances transcription elongation by stabilizing DNA base pairs immediately upstream of the RNA-DNA hybrid but does not measurably affect the nucleotide incorporation and the forward translocation by RNA polymerase.
84. 1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    An electrostatic selection mechanism controls sequential kinase signaling downstream of the T cell receptor

    Neel H Shah, Qi Wang ... John Kuriyan

    A high-throughput technique to characterize the substrate specificities of tyrosine kinases identifies the key features of kinases and substrates that enforce accurate signaling from T cell receptors.
85. 1. Biochemistry and Chemical Biology
    2. Plant Biology

    Limitations to photosynthesis by proton motive force-induced photosystem II photodamage

    Geoffry A Davis, Atsuko Kanazawa ... David M Kramer

    Storage of solar energy in the thylakoid electrical field by photosynthesis in vivo can substantially destabilize charge-separated states in photosystem II, leading to singlet oxygen production and photodamage, contributing to loss of productivity, especially under fluctuating light experienced in the field.
86. 1. Neuroscience

    Inhibiting poly(ADP-ribosylation) improves axon regeneration

    Alexandra B Byrne, Rebecca D McWhirter ... Marc Hammarlund

    In C. elegans and mouse neurons, the balance between poly(ADP-ribose) glycohydrolases and poly(ADP-ribose) polymerases regulates axon regeneration downstream of DLK-1/MAPKKK signaling.
87. 1. Computational and Systems Biology
    2. Developmental Biology

    Stochasticity in the miR-9/Hes1 oscillatory network can account for clonal heterogeneity in the timing of differentiation

    Nick E Phillips, Cerys S Manning ... Nancy Papalopulu

    Stochasticity introduced computationally into a gene expression oscillator creates heterogeneity in the time of differentiation of identical cells and offers robustness to the progenitor state and the outcome of cell division.
88. 1. Cancer Biology
    2. Cell Biology

    Rac1-mediated membrane raft localization of PI3K/p110β is required for its activation by GPCRs or PTEN loss

    Onur Cizmecioglu, Jing Ni ... Thomas M Roberts

    A unique two-step mechanism of plasma membrane recruitment and activation governs G-protein-coupled receptor mediated PI3K signaling.
89. 1. Neuroscience

    Social observation enhances cross-environment activation of hippocampal place cell patterns

    Xiang Mou, Daoyun Ji

    Place cells associated with track-running are activated in rats staying in a nearby box while another rat running on the track, suggesting that social observation facilitates spatial memory representations.
90. 1. Cell Biology
    2. Developmental Biology

    The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs

    Yifei Zhang, Xing Wang ... Seth S Blair

    The Fat-regulated scaffolding protein Dlish controls the activity, levels and cortical localization of Warts-inhibiting myosin Dachs by linking Dachs to a critical Dachs regulator, the DHHC palmitoyltransferase Approximated, and to the Fat-mediated regulation of Dachs stability.
91. 1. Cancer Biology

    Macrophage PPARγ inhibits Gpr132 to mediate the anti-tumor effects of rosiglitazone

    Wing Yin Cheng, HoangDinh Huynh ... Yihong Wan

    The PPARγ protein acts in macrophages to inhibit breast cancer progression and mediate the anti-tumor effects of rosiglitazone by suppressing Gpr132 – a pro-tumor and pro-inflammatory factor in macrophages.
92. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    ATPase activity of the DEAD-box protein Dhh1 controls processing body formation

    Christopher Frederick Mugler, Maria Hondele ... Karsten Weis

    The ATPase Dhh1 controls processing body formation and disassembly in yeast cells, and processing body dynamics can be recapitulated in vitro with recombinant Dhh1, ATP and RNA.
93. 1. Neuroscience

    Sparse activity of identified dentate granule cells during spatial exploration

    Maria Diamantaki, Markus Frey ... Andrea Burgalossi

    Morphologically-mature granule cells with complex dendritic trees contribute to a sparse representation of space in the rat dentate gyrus.
94. 1. Evolutionary Biology

    Phenotypic plasticity as an adaptation to a functional trade-off

    Xiao Yi, Antony M Dean

    A plastic behavior in Escherichia coli is enabled by a single amino acid replacement in adaption to changing environments.
95. 1. Chromosomes and Gene Expression

    The fail-safe mechanism of post-transcriptional silencing of unspliced HAC1 mRNA

    Rachael Di Santo, Soufiane Aboulhouda, David E Weinberg

    The unspliced HAC1 mRNA does not give rise to detectable protein in budding yeast, despite its cytoplasmic localization, due to a two-part post-transcriptional silencing mechanism.
96. 1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization

    Zhong-Yu Liu, Xiao-Feng Li ... Cheng-Feng Qin

    A conserved element in the flavivirus genomic 5′ terminus switches its conformation in response to long-range RNA interactions, and thereby regulates the dynamic recruitment of viral replicase for efficient viral RNA replication.
97. 1. Evolutionary Biology
    2. Neuroscience

    Evidence for evolutionary divergence of activity-dependent gene expression in developing neurons

    Jing Qiu, Jamie McQueen ... Giles E Hardingham

    The transcriptional response of human neurons to calcium ion signals shows evolutionary divergence from those responses elicited in mouse neurons, providing evidence in favour of using human systems to study neuronal responses to external stimuli.