230 results found
    1. Cell Biology

    Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism

    Sam Li, Jose-Jesus Fernandez ... David A Agard
    A detailed description of the structure of procentriole MT triplet by cryoET, along with its associated non-tubulin proteins and its assembly intermediates, reveals possible molecular mechanism for the procentriole assembly.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Molecular structures and conformations of protocadherin-15 and its complexes on stereocilia elucidated by cryo-electron tomography

    Johannes Elferich, Sarah Clark ... Eric Gouaux
    Direct imaging of individual molecules of the tip-link protein PCDH15 in mouse stereocilia shows that it assembles as a dimer, forms complexes that span stereocilia, and occurs in clusters with multiple copies of the PCDH15 dimer.
    1. Structural Biology and Molecular Biophysics

    A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0

    Jasenko Zivanov, Joaquín Otón ... Sjors HW Scheres
    New functionality in RELION-4.0 allows convenient structure determination from cryo electron tomography data to resolutions sufficient for de novo atomic modelling.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Cryo electron tomography with volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen Trypanosoma brucei

    Simon Imhof, Jiayan Zhang ... Kent L Hill
    Cryo electron tomography provides the first high-resolution 3D axoneme structure from any pathogenic organism, revealing novel structures that support the unique motility of these pathogens through host tissues.
    1. Structural Biology and Molecular Biophysics
    2. Plant Biology

    Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography

    Benjamin D Engel, Miroslava Schaffer ... Wolfgang Baumeister
    The first 3D views of the native algal chloroplast provide new insights into thylakoid biogenesis, photosynthesis, and carbon fixation.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling

    C Keith Cassidy, Benjamin A Himes ... Peijun Zhang
    An atomic model of the bacterial chemosensory array obtained through the synthesis of cryo-electron tomography and large-scale molecular-dynamics simulations reveals a new kinase conformation during signaling events.
    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    In situ cryo-ET structure of phycobilisome–photosystem II supercomplex from red alga

    Meijing Li, Jianfei Ma ... Sen-Fang Sui
    The nature of the phycobilisome–photosystem II supercomplex on the native thylakoid determined with cryo-electron tomography at an unprecedented resolution reveals that one phycobilisome interconnects with six photosystem monomers.
    1. Structural Biology and Molecular Biophysics

    Fully automated, sequential focused ion beam milling for cryo-electron tomography

    Tobias Zachs, Andreas Schertel ... Martin Pilhofer
    The automation of cryo-focused ion beam milling enables previously unfeasible cryo-electron tomography projects.
    1. Microbiology and Infectious Disease

    Cryo-electron tomography reveals novel features of a viral RNA replication compartment

    Kenneth J Ertel, Desirée Benefield ... Paul Ahlquist
    Cryo-electron tomography unveils striking new structural components of positive-strand virus RNA replication compartments, greatly advancing mechanistic insights into the structure, assembly, function and control of these critical complexes.
    1. Cell Biology

    Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids

    Patrick C Hoffmann, Stefano L Giandomenico ... Wanda Kukulski
    Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.

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