A detailed description of the structure of procentriole MT triplet by cryoET, along with its associated non-tubulin proteins and its assembly intermediates, reveals possible molecular mechanism for the procentriole assembly.
Direct imaging of individual molecules of the tip-link protein PCDH15 in mouse stereocilia shows that it assembles as a dimer, forms complexes that span stereocilia, and occurs in clusters with multiple copies of the PCDH15 dimer.
New functionality in RELION-4.0 allows convenient structure determination from cryo electron tomography data to resolutions sufficient for de novo atomic modelling.
Cryo electron tomography provides the first high-resolution 3D axoneme structure from any pathogenic organism, revealing novel structures that support the unique motility of these pathogens through host tissues.
C Keith Cassidy, Benjamin A Himes ... Peijun Zhang
An atomic model of the bacterial chemosensory array obtained through the synthesis of cryo-electron tomography and large-scale molecular-dynamics simulations reveals a new kinase conformation during signaling events.
The nature of the phycobilisome–photosystem II supercomplex on the native thylakoid determined with cryo-electron tomography at an unprecedented resolution reveals that one phycobilisome interconnects with six photosystem monomers.
Kenneth J Ertel, Desirée Benefield ... Paul Ahlquist
Cryo-electron tomography unveils striking new structural components of positive-strand virus RNA replication compartments, greatly advancing mechanistic insights into the structure, assembly, function and control of these critical complexes.
Patrick C Hoffmann, Stefano L Giandomenico ... Wanda Kukulski
Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.