Duplication of Leishmania chromosomes combines S-phase DNA replication initiated at a single internal region with subtelomeric DNA replication detectable outside S-phase, potentially explaining genome plasticity in this important parasite.

Evolutionary adaptation to a constitutive perturbation of DNA replication reveals that adaptive mutations in three conserved pathways interact to restore faithful chromosome replication and segregation.

Biochemical and biological characterization of insulator complex SUMM4 reveals that, in addition to disrupting enhancer–promoter interactions and establishing chromatin barriers, it functions to delay DNA replication, thus implicating architectural proteins in shaping spatiotemporal patterns of replication.

Human cell lines replicate and proliferate without ORC1 or ORC2, two subunits of the replication initiator protein complex ORC, which has till now been considered essential for DNA replication.

The intrinsically disordered regions (IDRs) of metazoan DNA replication licensing factors phase separate through synergistic electrostatic DNA-IDR and hydrophobic inter-IDR interactions.

Single-particle cryo-EM structures reveal a multistep process of how DNA is loaded into the DNA sliding clamp proliferating cell nuclear antigen by the canonical clamp loader replication factor C.

During tumorigenesis loss of p53 not only abrogates cell cycle arrest and apoptosis, but also suppresses the induction of replication-stress-induced DNA double-stranded breaks.

Two related DNA replication initiation proteins contribute to the decision of whether to enter a new round of the cell division cycle or enter into a period of proliferative quiescence.

The ~20,000 origins of replication in human cell lines that are reproducibly identified by multiple techniques in multiple cell lines are distant from known origin recognition complex and MCM2-7-binding sites.

In contrast to other transcription factors, CTCF and Esrrb rapidly regain binding after replication and remain bound to their targets during mitosis, preserving local nucleosome organization throughout the cell cycle.