The conductance-based encoding model creates a new bridge between statistical models and biophysical models of neurons, and infers visually-evoked excitatory and inhibitory synaptic conductances from spike trains in macaque retina.
Monarin Uervirojnangkoorn, Oliver B Zeldin ... William I Weis
Post-refinement methods enable the use of X-ray free electron lasers for biological crystallography of systems in which sample quantity is a limitation.
Quantitative FRET imaging in moving C. elegans shows that stretch-unfolding of twitchin kinase occurs in the active muscle, whereby mechanical activity titrates the signaling pathway of this cytoskeletal kinase.
A computational model, based on single-cell features like contractility and polarizability, quantitatively describes cellular dynamics from the single cell level up to small cohorts and confluent tissues.
Johannes Thomsen, Magnus Berg Sletfjerding ... Nikos S Hatzakis
An open-source user-friendly toolbox implementing machine learning for single-molecule FRET analysis enabling experts and non-experts to reproducibly provide dynamic structural biology insights.
The tetrameric structure of a casposase bound to DNA and its biochemical properties show how a transposase could have evolved to perform CRISPR-Cas spacer acquisition.