14,797 results found
    1. Cell Biology

    Two subunits of human ORC are dispensable for DNA replication and proliferation

    Etsuko Shibata, Manjari Kiran ... Anindya Dutta
    Human cell lines replicate and proliferate without ORC1 or ORC2, two subunits of the replication initiator protein complex ORC, which has till now been considered essential for DNA replication.
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    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation

    Franziska Bleichert, Maxim Balasov ... James M Berger
    Electron microscopy uncovers the structure of the origin recognition complex (ORC) in metazoans, and reveals how mutations in the ORC6 subunit lead to Meier-Gorlin syndrome in humans.
    1. Chromosomes and Gene Expression

    Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones

    Nina Kirstein, Alexander Buschle ... Aloys Schepers
    Replication origins are established throughout the genome with the exception of transcribed genes, and the local chromatin composition likely modulates the density of ORC and MCM as well as origin activation.
    1. Chromosomes and Gene Expression

    The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure

    Hsiang-Chen Chou, Kuhulika Bhalla ... Bruce Stillman
    The initiation of human genome replication requires the six-subunit origin recognition complex (ORC) and CDC6, with ORC playing additional roles during mitosis and in organization of the cell nucleus.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

    Sumanprava Giri, Vasudha Aggarwal ... Supriya G Prasanth
    Origin recognition complex-associated (ORCA) is crucial for the stability of the Histone H3 lysine 9 methyltransferase megacomplex, which is essential for heterochromatin organization.
    1. Chromosomes and Gene Expression

    The dynamic nature of the human origin recognition complex revealed through five cryoEM structures

    Matt J Jaremko, Kin Fan On ... Leemor Joshua-Tor
    The origin recognition complex is a dynamic complex that assumes various conformational states that likely correspond to different steps in replication initiation.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    A helicase-tethered ORC flip enables bidirectional helicase loading

    Shalini Gupta, Larry J Friedman ... Stephen P Bell
    A single origin–recognition complex (ORC) directs loading of a pair of head-to-head Mcm2-7 replicative DNA helicases by forming a protein tether to the first helicase, releasing from its initial DNA-binding site, and rebinding the origin DNA in the opposite orientation.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Structure of the active form of human origin recognition complex and its ATPase motor module

    Ante Tocilj, Kin Fan On ... Leemor Joshua-Tor
    The human Origin Replication Complex is shaped as a shallow corkscrew in a classic AAA+ organization reminiscent of clamp loader complexes with highly controlled ATPase activity as exemplified by Meier-Gorlin syndrome mutations.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription

    Manzar Hossain, Bruce Stillman
    Two related DNA replication initiation proteins contribute to the decision of whether to enter a new round of the cell division cycle or enter into a period of proliferative quiescence.
    1. Structural Biology and Molecular Biophysics

    Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7

    Fuzhou Ye, Ioly Kotta-Loizou ... Xiaodong Zhang
    DNA mimicry Ocr protein, a well-studied T7 phage protein that inhibits host restriction enzymes, can also inhibit host transcription through competing with sigma factors in binding to RNA polymerase.

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