Raymond F Pauszek III, Rajan Lamichhane ... David P Millar
The primer extension and 5' flap processing activities of DNA polymerase I are physically coordinated by combined movements of the DNA substrate and the 5' nuclease domain of the enzyme.
Lilia Leisle, Rahul Chadda ... Christopher A Ahern
Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
Arc-shaped PFO oligomers consisting of at least four subunits can insert into the membrane in a kinetically controlled process opening a transmembrane pore, whereby post-insertion growth may ultimately lead to formation of a complete ring.
Martina Rangl, Nicolaus Schmandt ... Simon Scheuring
High-speed atomic force microscopy reveals that the open state of the divalent ion channel CorA is highly dynamic and defined by the fast exchange between conformations.
Reconstitution of DNA loop extrusion in cellular contexts using Xenopus egg extracts shows that condensin extrudes DNA loops non-symmetrically in metaphase, whereas cohesin extrudes DNA loops symmetrically in interphase.
The rate of DNA unwinding by RecQ helicases is dramatically modulated by the DNA duplex stability in a geometry-dependent manner, providing an intrinsic mechanism for suppressing illegitimate recombination.
Mathias L Heltberg, Judith Miné-Hattab ... Thierry Mora
Two competing descriptions of membrane-less sub-compartments, liquid droplets and polymer binding aggregates, can be cast into a common theoretical framework and tested through single-particle tracking experiments.
Specific and non-specific conformational confinement, via point mutation or cochaperone interaction or macro-molecular crowding, stimulates protein function in Hsp90 by reducing non-productive conformational flexibility.
Daniel R Larson, Christoph Fritzsch ... Robert H Singer
Steroid hormone receptors control the expression of their target genes through a digital on-off switch in individual cells, which leads to an analogue dose-response relationship at the level of the whole organism.
Nadia M Huisjes, Thomas M Retzer ... Karl E Duderstadt
Mars provides a software platform for the development of complex single-molecule analysis workflows enabling the discovery and reporting of new biological phenomena with an open data format accessible to everyone.
