Single molecule DNA-binding trajectories and deterministic modeling analyses demonstrate a functional role for high energy partly folded states in Transcription Activator-Like Effectors that could improve future TALEN design.

Arc-shaped PFO oligomers consisting of at least four subunits can insert into the membrane in a kinetically controlled process opening a transmembrane pore, whereby post-insertion growth may ultimately lead to formation of a complete ring.

Chromatin remodeling enzymes perform target search by utilizing ATP-binding and hydrolysis to facilitate 1D-diffusion on and rapid detachment from chromatin in living yeast.

Disassembly of the HIV-1 capsid is a catastrophic process, whereby initiation and propagation can be controlled independently by molecules that bind to different features of the capsid lattice.

The conformational dynamics of multidrug resistance protein 1 (MRP1) are tracked in real time by single-molecule fluorescence resonance energy transfer experiments, which elucidate the rate-limiting mechanism of MRP1's transport cycle.

The rate of DNA unwinding by RecQ helicases is dramatically modulated by the DNA duplex stability in a geometry-dependent manner, providing an intrinsic mechanism for suppressing illegitimate recombination.

The scanning-assisted clustering mechanism of IFI16 not only allows efficient assembly on exposed foreign-dsDNA, but also suppresses the engagement of chromatinized self-dsDNA.

The motility of three different kinesin-dynein pairs were analyzed and found to be strikingly similar, suggesting that bidirectional transport is not regulated by motor type, but rather by scaffold, adapter, and regulatory proteins.

The budding yeast transcription factors Reb1 and Cbf1 function as pioneer factors by slowly dissociating from nucleosomes, allowing them to target and unwrap nucleosomes efficiently to regulate transcription.

Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.