Transposition reactions that occur during DNA replication and involve the termini of adjacent transposons can induce genome expansion by re-replication of transposon-flanking sequences.
Biochemical data demonstrate that the conformation of the target site DNA can dramatically modulate the kinetics and directionality of the otherwise isoenergetic transposition reaction of DDE transposases.
Genetic, structural, and biochemical analyses of IS607-family transposons shows that the DNA translocation reaction proceeds very differently from other reactions promoted by serine recombinases.
Agnès H Michel, Riko Hatakeyama ... Benoît Kornmann
A new method maps functional and structural features of yeast genomes with unprecedented ease and throughput, which allows identification of protein domains at the genome scale.
Fusion proteins composed of the Sleeping Beauty transposase and catalytically inactive Cas9 target transposon integration into genomic regions specified by single guide RNAs.
The tetrameric structure of a casposase bound to DNA and its biochemical properties show how a transposase could have evolved to perform CRISPR-Cas spacer acquisition.
Disintegration, regarded as an abortive side reaction antithetical to DNA transposition, can promote protospacer integration at the CRISPR locus via DNA repair pathways.
Mathieu Hénault, Souhir Marsit ... Christian R Landry
The gain of transposable elements in yeast hybrids does not scale with genetic divergence levels and is most impacted by structural variations and multiple aspects of individual hybrid genetic backgrounds.
