MOF-associated complexes have overlapping and unique roles in regulating pluripotency in embryonic stem cells and during differentiation
Abstract
The histone acetyltransferase (HAT) Mof is essential for mouse embryonic stem cells (mESC) pluripotency and early development. Mof is the enzymatic subunit of two different HAT complexes, MSL and NSL. The individual contribution of MSL and NSL to transcription regulation in mESCs is not well understood. Our genome-wide analysis show that i) MSL and NSL bind to specific and common sets of expressed genes, ii) NSL binds exclusively at promoters, iii) while MSL binds in gene bodies. Nsl1 regulates proliferation and cellular homeostasis of mESCs. MSL is the main HAT acetylating H4K16 in mESCs, is enriched at many mESC-specific and bivalent genes. MSL is important to keep a subset of bivalent genes silent in mESCs, while developmental genes require MSL for expression during differentiation. Thus, NSL and MSL HAT complexes differentially regulate specific sets of expressed genes in mESCs and during differentiation.
Article and author information
Author details
Reviewing Editor
- Danny Reinberg, Howard Hughes Medical Institute, New York University School of Medicine, United States
Version history
- Received: December 17, 2013
- Accepted: June 3, 2014
- Accepted Manuscript published: June 4, 2014 (version 1)
- Version of Record published: June 17, 2014 (version 2)
Copyright
© 2014, Ravens et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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