1. Structural Biology and Molecular Biophysics
  2. Stem Cells and Regenerative Medicine

ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation

  1. Nataliya Nady
  2. Ankit Gupta
  3. Ziyang Ma
  4. Tomek Swigut
  5. Akiko Koide
  6. Shohei Koide
  7. Joanna Wysocka  Is a corresponding author
  1. Stanford University School of Medicine, United States
  2. University of Chicago, United States
https://doi.org/10.7554/eLife.10150
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  1. Nataliya Nady
  2. Ankit Gupta
  3. Ziyang Ma
  4. Tomek Swigut
  5. Akiko Koide
  6. Shohei Koide
  7. Joanna Wysocka
(2015)
ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation
eLife 4:e10150.
https://doi.org/10.7554/eLife.10150
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Abstract

Prdm14 is a sequence-specific transcriptional regulator of embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. It exerts its function, at least in part, through repressing genes associated with epigenetic modification and cell differentiation. Here, we show that this repressive function is mediated through an ETO-family co-repressor Mtgr1, which tightly binds to the pre-SET/SET domains of Prdm14 and co-occupies its genomic targets in mouse ESCs. We generated two monobodies, synthetic binding proteins, targeting the Prdm14 SET domain and demonstrate their utility, respectively, in facilitating crystallization and structure determination of the Prdm14-Mtgr1 complex, or as genetically encoded inhibitor of the Prdm14-Mtgr1 interaction. Structure-guided point mutants and the monobody abrogated the Prdm14-Mtgr1 association and disrupted Prdm14's function in mESC gene expression and PGC formation in vitro. Altogether, our work uncovers the molecular mechanism underlying Prdm14-mediated repression and provides renewable reagents for studying and controlling Prdm14 functions.

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Author details

  1. Nataliya Nady

    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, United States
    Competing interests
    No competing interests declared.

  2. Ankit Gupta

    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, United States
    Competing interests
    Ankit Gupta, SK and AK are inventors on a patent application filed by the University of Chicago that covers monobody library design (US 13/813,409).The monobodies described in this work are available from SK under a material transfer agreement with the University of Chicago..

  3. Ziyang Ma

    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, United States
    Competing interests
    No competing interests declared.

  4. Tomek Swigut

    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, United States
    Competing interests
    No competing interests declared.

  5. Akiko Koide

    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, United States
    Competing interests
    No competing interests declared.

  6. Shohei Koide

    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, United States
    Competing interests
    Shohei Koide, SK and AK are inventors on a patent application filed by the University of Chicago that covers monobody library design (US 13/813,409).The monobodies described in this work are available from SK under a material transfer agreement with the University of Chicago..

  7. Joanna Wysocka

    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, United States
    For correspondence
    wysocka@stanford.edu
    Competing interests
    No competing interests declared.

Reviewing Editor

  1. Karen Adelman, National Institute of Environmental Health Sciences, United States

Version history

  1. Received: July 20, 2015
  2. Accepted: November 1, 2015
  3. Accepted Manuscript published: November 2, 2015 (version 1)
  4. Version of Record published: February 1, 2016 (version 2)

Copyright

© 2015, Nady et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Nataliya Nady
  2. Ankit Gupta
  3. Ziyang Ma
  4. Tomek Swigut
  5. Akiko Koide
  6. Shohei Koide
  7. Joanna Wysocka
(2015)
ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation
eLife 4:e10150.
https://doi.org/10.7554/eLife.10150

Share this article

https://doi.org/10.7554/eLife.10150

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