The carcinine transporter CarT is required in Drosophila photoreceptor neurons to sustain histamine recycling
Abstract
Synaptic transmission from Drosophila photoreceptors to lamina neurons requires recycling of histamine neurotransmitter. Synaptic histamine is cleared by uptake into glia and conversion into carcinine, which functions as transport metabolite. How carcinine is transported from glia to photoreceptor neurons remains unclear. In a targeted RNAi screen for genes involved in this pathway we identified carT, which encodes a member of the SLC22A transporter family. CarT expression in photoreceptors is necessary and sufficient for fly vision and behavior. In the lamina of carT flies, carcinine accumulates. Wild-type levels are restored by photoreceptor-specific expression of CarT, and endogenous tagging suggests CarT localizes to synaptic endings. Heterologous expression of CarT in S2 cells is sufficient for carcinine uptake, demonstrating the ability of CarT to utilize carcinine as a transport substrate. Together, our results demonstrate that CarT transports the histamine metabolite carcinine into photoreceptor neurons, thus contributing an essential step in the histamine-carcinine cycle.
Article and author information
Author details
Reviewing Editor
- Hugo J Bellen, Howard Hughes Medical Institute, Baylor College of Medicine, United States
Version history
- Received: August 19, 2015
- Accepted: December 13, 2015
- Accepted Manuscript published: December 14, 2015 (version 1)
- Version of Record published: January 25, 2016 (version 2)
Copyright
© 2015, Stenesen et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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