Sensitive red protein calcium indicators for imaging neural activity
- Howard Hughes Medical Institute, United States
- Howard Hughes Medical Institute, The Rockefeller University, United States
Abstract
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.
Article and author information
Author details
Reviewing Editor
- Michael Häusser, University College London, United Kingdom
Ethics
Animal experimentation: All experimental protocols were conducted according to NationalInstitutes of Health guidelines for animal research and were approved bythe Institutional Animal Care and Use Committee at Janelia ResearchCampus (protocol 13-95).
Version history
- Received: November 1, 2015
- Accepted: March 24, 2016
- Accepted Manuscript published: March 24, 2016 (version 1)
- Version of Record published: April 26, 2016 (version 2)
Copyright
© 2016, Dana et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Sensitive red protein calcium indicators for imaging neural activity
eLife 5:e12727.
https://doi.org/10.7554/eLife.12727
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