Primate TRIM5 proteins form hexagonal nets 1 on HIV-1 capsids
- University of Utah, United States
- California Institute of Technology, United States
- University of Virginia, United States
Abstract
TRIM5 proteins are restriction factors that block retroviral infections by binding viral capsids and preventing reverse transcription. Capsid recognition is mediated by C-terminal domains on TRIM5α (SPRY) or TRIMCyp (cyclophilin A), which interact weakly with capsids. Efficient capsid recognition also requires the conserved N-terminal tripartite motifs (TRIM), which mediate oligomerization and create avidity effects. To characterize how TRIM5 proteins recognize viral capsids, we developed methods for isolating native recombinant TRIM5 proteins and purifying stable HIV-1 capsids. Biochemical and EM analyses revealed that TRIM5 proteins assembled into hexagonal nets, both alone and on capsid surfaces. These nets comprised open hexameric rings, with the SPRY domains centered on the edges and the B-box and RING domains at the vertices. Thus, the principles of hexagonal TRIM5 assembly and capsid pattern recognition are conserved across primates, allowing TRIM5 assemblies to maintain the conformational plasticity necessary to recognize divergent and pleomorphic retroviral capsids.
Article and author information
Author details
Reviewing Editor
- Stephen P Goff, Howard Hughes Medical Institute, Columbia University, United States
Version history
- Received: March 22, 2016
- Accepted: May 19, 2016
- Accepted Manuscript published: June 2, 2016 (version 1)
- Accepted Manuscript updated: June 3, 2016 (version 2)
- Version of Record published: July 7, 2016 (version 3)
Copyright
© 2016, Li et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Primate TRIM5 proteins form hexagonal nets 1 on HIV-1 capsids
eLife 5:e16269.
https://doi.org/10.7554/eLife.16269
Further reading
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- Structural Biology and Molecular Biophysics
- Microbiology and Infectious Disease
Structural studies reveal how an antiviral factor forms a molecular net to restrict retroviruses including HIV-1.
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- Developmental Biology
- Immunology and Inflammation
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