1. Neuroscience

Subretinal mononuclear phagocytes induce cone segment loss via IL-1β

  1. Chiara M Eandi
  2. Hugo Charles Messance
  3. Sébastien Augustin
  4. Elisa Dominguez
  5. Sophie Lavalette
  6. Valérie Forster  Is a corresponding author
  7. Shulong Justin Hu
  8. Lourdes Siquieros
  9. Cheryl Mae Craft
  10. José-Alain Sahel
  11. Ramin Tadayoni
  12. Michel Paques
  13. Xavier Guillonneau
  14. Florian Sennlaub  Is a corresponding author
  1. Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, France
  2. Keck School of Medicine of the University of Southern California, United States
https://doi.org/10.7554/eLife.16490
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  1. Chiara M Eandi
  2. Hugo Charles Messance
  3. Sébastien Augustin
  4. Elisa Dominguez
  5. Sophie Lavalette
  6. Valérie Forster
  7. Shulong Justin Hu
  8. Lourdes Siquieros
  9. Cheryl Mae Craft
  10. José-Alain Sahel
  11. Ramin Tadayoni
  12. Michel Paques
  13. Xavier Guillonneau
  14. Florian Sennlaub
(2016)
Subretinal mononuclear phagocytes induce cone segment loss via IL-1β
eLife 5:e16490.
https://doi.org/10.7554/eLife.16490
  2. Download BibTeX
  3. Download .RIS

Abstract

Photo-transduction in cone segments (CS) is crucial for high acuity daytime vision. For ill-defined reasons, CS degenerate in retinitis pigmentosa (RP) and in the transitional zone (TZ) of atrophic zones (AZ), which characterize geographic atrophy (GA). Our experiments confirm the loss of cone segments (CS) in the TZ of GA patients and show their association with subretinal CD14+mononuclear phagocyte (MP) infiltration that is also reported in RP. Using human and mouse MPs in vitro and inflammation-prone Cx3cr1GFP/GFP mice in vivo, we demonstrate that MP derived IL-1β leads to severe CS degeneration. Our results strongly suggest that subretinal MP accumulation participates in the observed pathological photoreceptor changes in these diseases. Inhibiting subretinal MP accumulation or Il-1β might protect the CS and help preserve high acuity daytime vision in conditions characterized by subretinal inflammation, such as AMD and RP.

Article and author information

Author details

  1. Chiara M Eandi

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3656-1689

  2. Hugo Charles Messance

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  3. Sébastien Augustin

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  4. Elisa Dominguez

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  5. Sophie Lavalette

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  6. Valérie Forster

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    For correspondence
    valerie.fradot@inserm.fr
    Competing interests
    The authors declare that no competing interests exist.

  7. Shulong Justin Hu

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  8. Lourdes Siquieros

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  9. Cheryl Mae Craft

    Mary D. Allen Laboratory for Vision Research, Keck School of Medicine of the University of Southern California, Los ANgeles, United States
    Competing interests
    The authors declare that no competing interests exist.

  10. José-Alain Sahel

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  11. Ramin Tadayoni

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  12. Michel Paques

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.

  13. Xavier Guillonneau

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7379-3935

  14. Florian Sennlaub

    Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Paris, France
    For correspondence
    florian.sennlaub@inserm.fr
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4412-1341

Funding

Agence Nationale de la Recherche (MACLEAR)

  • Sébastien Augustin
  • Elisa Dominguez
  • Sophie Lavalette
  • Shulong Justin Hu
  • Lourdes Siquieros
  • José-Alain Sahel
  • Michel Paques
  • Xavier Guillonneau
  • Florian Sennlaub

Labex (lifescience)

  • Chiara M Eandi
  • Hugo Charles Messance
  • Sébastien Augustin
  • Elisa Dominguez
  • Sophie Lavalette
  • Shulong Justin Hu
  • Lourdes Siquieros
  • José-Alain Sahel
  • Ramin Tadayoni
  • Michel Paques
  • Xavier Guillonneau
  • Florian Sennlaub

adps-allianz

  • Chiara M Eandi
  • Hugo Charles Messance
  • Sébastien Augustin
  • Elisa Dominguez
  • Sophie Lavalette
  • Shulong Justin Hu
  • Lourdes Siquieros
  • José-Alain Sahel
  • Ramin Tadayoni
  • Michel Paques
  • Xavier Guillonneau
  • Florian Sennlaub

National Institutes of Health (EY015851)

  • Cheryl Mae Craft

National Institutes of Health (EY03040)

  • Cheryl Mae Craft

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Jeremy Nathans, Johns Hopkins University School of Medicine, United States

Ethics

Animal experimentation: All experimental protocols and procedures were approved by the local animal care ethics committee (00156.02; C 92-032-02 )

Human subjects: Volunteers provided written and informed consent for the human monocyte expression studies, which were approved by the Centre national d'ophthalmologie des Quinze-Vingt hospital (Paris, France) ethics committees (no. 913572)

Version history

  1. Received: April 2, 2016
  2. Accepted: July 19, 2016
  3. Accepted Manuscript published: July 20, 2016 (version 1)
  4. Version of Record published: August 1, 2016 (version 2)

Copyright

© 2016, Eandi et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Chiara M Eandi
  2. Hugo Charles Messance
  3. Sébastien Augustin
  4. Elisa Dominguez
  5. Sophie Lavalette
  6. Valérie Forster
  7. Shulong Justin Hu
  8. Lourdes Siquieros
  9. Cheryl Mae Craft
  10. José-Alain Sahel
  11. Ramin Tadayoni
  12. Michel Paques
  13. Xavier Guillonneau
  14. Florian Sennlaub
(2016)
Subretinal mononuclear phagocytes induce cone segment loss via IL-1β
eLife 5:e16490.
https://doi.org/10.7554/eLife.16490

Share this article

https://doi.org/10.7554/eLife.16490

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