Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4
- Harvard University, United States
- Harvard Medical School, United States
Abstract
Protocadherins (Pcdhs) are cell adhesion and signaling proteins used by neurons to develop and maintain neuronal networks, relying on trans homophilic interactions between their extracellular cadherin (EC) repeat domains. We present the structure of the antiparallel EC1-4 homodimer of human PcdhγB3, a member of the γ subfamily of clustered Pcdhs. Structure and sequence comparisons of α, β, and γ clustered Pcdh isoforms illustrate that subfamilies encode specificity in distinct ways through diversification of loop region structure and composition in EC2 and EC3, which contains isoform-specific conservation of primarily polar residues. In contrast, the EC1/EC4 interface comprises hydrophobic interactions that provide non-selective dimerization affinity. Using sequence coevolution analysis, we found evidence for a similar antiparallel EC1-4 interaction in non-clustered Pcdh families. We thus deduce that the EC1-4 antiparallel homodimer is a general interaction strategy that evolved before the divergence of these distinct protocadherin families.
Data availability
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Structure of human clustered protocadherin gamma B3 EC1-4Publicly available at the RCSB Protein Data Bank (accession no: 5K8R).
Article and author information
Author details
Funding
National Science Foundation
- Anna G Green
National Institutes of Health (NCRR 1S10RR028832-01)
- Debora S Marks
National Institute of General Medical Sciences (GM106303)
- Debora S Marks
National Defense Science and Engineering Graduate Fellowship (DGE1144152)
- John M Nicoludis
National Institute of General Medical Sciences (P41 GM103403)
- Rachelle Gaudet
National Institutes of Health (S10 RR029205)
- Rachelle Gaudet
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- William I Weis, Stanford University, United States
Version history
- Received: June 6, 2016
- Accepted: July 28, 2016
- Accepted Manuscript published: July 29, 2016 (version 1)
- Version of Record published: August 26, 2016 (version 2)
Copyright
© 2016, Nicoludis et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4
eLife 5:e18449.
https://doi.org/10.7554/eLife.18449
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