IL17 factors are early regulators in the gut epithelium during inflammatory response to Vibrio in the sea urchin larva
- The George Washington University, United States
- Sunnybrook Research Institute, Canada
- Saitama University, Japan
- University of Toronto, Canada
Abstract
IL17 cytokines are central mediators of mammalian immunity. In vertebrates, these factors derive from diverse cellular sources. Sea urchins share a molecular heritage with chordates that includes the IL17 system. Here, we characterize the role of epithelial expression of IL17 in the larval gut-associated immune response. The purple sea urchin genome encodes ten IL17 subfamilies (35 genes) and two IL17 receptors. Most of these subfamilies are conserved throughout echinoderms. Two IL17 subfamilies are sequentially strongly upregulated and attenuated in the gut epithelium in response to bacterial disturbance. IL17R1 signal perturbation results in reduced expression of several response genes including an IL17 subtype, indicating a potential feedback. A third IL17 subfamily is activated in adult immune cells indicating that expression in immune cells and epithelia is divided among families. The larva provides a tractable model to investigate the regulation and consequences of gut epithelial IL17 expression across the organism.
Data availability
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Strongylocentrotus purpuratus developmental transcriptomesPublicly available at the NCBI Gene Expression Omnibus (accession no: PRJNA81157).
Article and author information
Author details
Katherine M Buckley
Jonathan Patrick Rast
Funding
Canadian Institutes of Health Research (MOP74667)
- Jonathan Patrick Rast
Natural Sciences and Engineering Research Council of Canada (312221)
- Jonathan Patrick Rast
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Sarah Gaffen
Ethics
Animal experimentation: All animal care and use protocols were approved by the Sunnybrook Research Institute Animal Care Committee
Version history
- Received: November 19, 2016
- Accepted: April 25, 2017
- Accepted Manuscript published: April 27, 2017 (version 1)
- Version of Record published: June 2, 2017 (version 2)
Copyright
© 2017, Buckley et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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IL17 factors are early regulators in the gut epithelium during inflammatory response to Vibrio in the sea urchin larva
eLife 6:e23481.
https://doi.org/10.7554/eLife.23481
Further reading
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The sea urchin may be a good model for understanding how immune responses work in humans and other vertebrates.
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