1. Developmental Biology
  2. Neuroscience

A novel perivascular cell population in the zebrafish brain

  1. Marina Venero Galanternik
  2. Daniel Castranova
  3. Aniket V Gore
  4. Nathan H Blewett
  5. Hyun Min Jung
  6. Amber N Stratman
  7. Martha R Kirby
  8. James Iben
  9. Mayumi F Miller
  10. Koichi Kawakami
  11. Richard J Maraia
  12. Brant M Weinstein  Is a corresponding author
  1. National Institutes of Health, United States
  2. National Institute of Genetics, Japan
https://doi.org/10.7554/eLife.24369
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  1. Marina Venero Galanternik
  2. Daniel Castranova
  3. Aniket V Gore
  4. Nathan H Blewett
  5. Hyun Min Jung
  6. Amber N Stratman
  7. Martha R Kirby
  8. James Iben
  9. Mayumi F Miller
  10. Koichi Kawakami
  11. Richard J Maraia
  12. Brant M Weinstein
(2017)
A novel perivascular cell population in the zebrafish brain
eLife 6:e24369.
https://doi.org/10.7554/eLife.24369
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Abstract

The blood-brain barrier is essential for the proper homeostasis and function of the CNS, but its mechanism of function is poorly understood. Perivascular cells surrounding brain blood vessels are thought to be important for blood-brain barrier establishment, but their roles are not well defined. Here, we describe a novel perivascular cell population closely associated with blood vessels on the zebrafish brain. Based on similarities in their morphology, location, and scavenger behavior, these cells appear to be the zebrafish equivalent of cells variably characterized as Fluorescent Granular Perithelial cells (FGPs), perivascular macrophages, or 'Mato Cells' in mammals. Despite their macrophage-like morphology and perivascular location, zebrafish FGPs appear molecularly most similar to lymphatic endothelium, and our imaging studies suggest that these cells emerge by transdifferentiation from endothelium of the optic choroidal vascular plexus. Our findings provide the first report of a perivascular cell population in the brain derived from vascular endothelium.

Article and author information

Author details

  1. Marina Venero Galanternik

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  2. Daniel Castranova

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  3. Aniket V Gore

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  4. Nathan H Blewett

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  5. Hyun Min Jung

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  6. Amber N Stratman

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  7. Martha R Kirby

    Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  8. James Iben

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  9. Mayumi F Miller

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  10. Koichi Kawakami

    Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-9993-1435

  11. Richard J Maraia

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    Competing interests
    The authors declare that no competing interests exist.

  12. Brant M Weinstein

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States
    For correspondence
    flyingfish2@nih.gov
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4136-4962

Funding

Eunice Kennedy Shriver National Institute of Child Health and Human Development (Z1A HD008808)

  • Marina Venero Galanternik
  • Daniel Castranova
  • Aniket V Gore
  • Hyun Min Jung
  • Amber N Stratman
  • Mayumi F Miller
  • Brant M Weinstein

National Human Genome Research Institute

  • Martha R Kirby

Japan Agency for Medical Research and Development

  • Koichi Kawakami

Japan Society for the Promotion of Science (JP15H02370 / JP16H01651)

  • Koichi Kawakami

Eunice Kennedy Shriver National Institute of Child Health and Human Development (Z1A HD000412-30)

  • Nathan H Blewett
  • James Iben
  • Richard J Maraia

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Didier YR Stainier, Max Planck Institute for Heart and Lung Research, Germany

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (ASP # 12-039 and 15-017).

Version history

  1. Received: December 20, 2016
  2. Accepted: March 28, 2017
  3. Accepted Manuscript published: April 11, 2017 (version 1)
  4. Version of Record published: May 9, 2017 (version 2)

Copyright

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

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  1. Marina Venero Galanternik
  2. Daniel Castranova
  3. Aniket V Gore
  4. Nathan H Blewett
  5. Hyun Min Jung
  6. Amber N Stratman
  7. Martha R Kirby
  8. James Iben
  9. Mayumi F Miller
  10. Koichi Kawakami
  11. Richard J Maraia
  12. Brant M Weinstein
(2017)
A novel perivascular cell population in the zebrafish brain
eLife 6:e24369.
https://doi.org/10.7554/eLife.24369

Share this article

https://doi.org/10.7554/eLife.24369

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