An inhibitory gate for state transition in cortex
- Italian Institute of Technology, Italy
Abstract
Large scale transitions between active (up) and silent (down) states during quiet wakefulness or NREM sleep regulate fundamental cortical functions and are known to involve both excitatory and inhibitory cells. However, if and how inhibition regulates these activity transitions is unclear. Using fluorescence-targeted electrophysiological recording and cell-specific optogenetic manipulation in both anesthetized and non-anesthetized mice, we found that two major classes of interneurons, the parvalbumin and the somatostatin positive cells, tightly control both up-to-down and down-to-up state transitions. Inhibitory regulation of state transition was found under both natural and optogenetically-evoked conditions, and was interneuron-type specific. Moreover, local manipulation of small ensembles of interneurons affected cortical populations millimetres away from the modulated region. Together, these results demonstrate that inhibition potently gates transitions between cortical activity states, and reveal the cellular mechanisms by which local inhibitory microcircuits regulate state transitions at the mesoscale.
Article and author information
Author details
Valentina Pasquale
Funding
European Research Council (NEURO-PATTERNS)
- Tommaso Fellin
National Institutes of Health (1U01NS090576-01)
- Tommaso Fellin
Seventh Framework Programme (DESIRE)
- Tommaso Fellin
MIUR FIRB (RBAP11X42L)
- Tommaso Fellin
Flag-Era JTC Human Brain Project (SLOW-DYN)
- Stefano Panzeri
- Tommaso Fellin
H2020 MSCA IF 2015: Manuel Molano ETIC (699829)
- Manuel Molano-Mazón
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- John Huguenard, Stanford University School of Medicine, United States
Ethics
Animal experimentation: Experimental procedures involving animals have been approved by the IIT Animal Welfare Body and by the Italian Ministry of Health (authorization # 34/2015-PR and 125/2012-B), in accordance with the National legislation (D.Lgs. 26/2014) and the European legislation (European Directive 2010/63/EU). All surgery was performed under urethane or isofluorane anesthesia, and every effort was made to minimize suffering.
Version history
- Received: February 21, 2017
- Accepted: May 15, 2017
- Accepted Manuscript published: May 16, 2017 (version 1)
- Version of Record published: May 25, 2017 (version 2)
Copyright
© 2017, Zucca et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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An inhibitory gate for state transition in cortex
eLife 6:e26177.
https://doi.org/10.7554/eLife.26177
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