Tuning self-renewal in the Arabidopsis stomatal lineage by hormone and nutrient regulation of asymmetric cell division
Abstract
Asymmetric and self-renewing divisions build and pattern tissues. In the Arabidopsis stomatal lineage, asymmetric cell divisions, guided by polarly localized cortical proteins, generate most cells on the leaf surface. Systemic and environmental signals modify tissue development, but the mechanisms by which plants incorporate such cues to regulate asymmetric divisions are elusive. In a screen for modulators of cell polarity, we identified CONSTITUTIVE TRIPLE RESPONSE1, a negative regulator of ethylene signaling. We subsequently revealed antagonistic impacts of ethylene and glucose signaling on the self-renewing capacity of stomatal lineage stem-cells. Quantitative analysis of cell polarity and fate dynamics showed that developmental information may be encoded in both the spatial and temporal asymmetries of polarity proteins. These results provide a framework for a mechanistic understanding of how nutritional status and environmental factors tune stem-cell behavior in the stomatal lineage, ultimately enabling flexibility in leaf size and cell-type composition.
Data availability
All data generated on analyzed during this study are include in the manuscript and supporting files.
Article and author information
Author details
Funding
Howard Hughes Medical Institute
- Dominique C Bergmann
Swiss national science foundation (EPM-PBLAP3-142757)
- Julien Alassimone
European Molecular Biology Organization (ALTF-878-2013)
- Julien Alassimone
Howard Hughes Medical Institute
- Yan Gong
Howard Hughes Medical Institute
- Nidhi Sharma
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Hao Yu, National University of Singapore & Temasek Life Sciences Laboratory, Singapore
Version history
- Received: September 22, 2020
- Accepted: March 18, 2021
- Accepted Manuscript published: March 19, 2021 (version 1)
- Version of Record published: March 30, 2021 (version 2)
Copyright
© 2021, Gong et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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