Sec17/Sec18 can support membrane fusion without help from completion of SNARE zippering
- Geisel School of Medicine at Dartmouth, United States
- Stanford University, United States
Abstract
Membrane fusion requires R-, Qa-, Qb-, and Qc-family SNAREs that zipper into RQaQbQc coiled coils, driven by the sequestration of apolar amino acids. Zippering has been thought to provide all the force driving fusion. Sec17/aSNAP can form an oligomeric assembly with SNAREs with the Sec17 C-terminus bound to Sec18/NSF, the central region bound to SNAREs, and a crucial apolar loop near the N-terminus poised to insert into membranes. We now report that Sec17 and Sec18 will drive robust fusion without requiring zippering completion. Zippering-driven fusion is blocked by deleting the C-terminal quarter of any Q-SNARE domain or by replacing the apolar amino acids of the Qa-SNARE which face the center of the 4-SNARE coiled coils with polar residues. These blocks, singly or combined, are bypassed by Sec17 and Sec18, and SNARE-dependent fusion is restored without help from completing zippering.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 1, 2, 3 4, 5, and 6.
Article and author information
Author details
Funding
National Institutes of Health (R35GM118037)
- William T Wickner
National Institutes of Health (R37MH63105)
- Axel T Brunger
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Josep Rizo, University of Texas Southwestern Medical Center, United States
Version history
- Received: February 16, 2021
- Accepted: April 30, 2021
- Accepted Manuscript published: May 4, 2021 (version 1)
- Version of Record published: May 24, 2021 (version 2)
Copyright
© 2021, Song et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Sec17/Sec18 can support membrane fusion without help from completion of SNARE zippering
eLife 10:e67578.
https://doi.org/10.7554/eLife.67578
Further reading
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- Biochemistry and Chemical Biology
Two proteins called Sec17 and Sec18 may have a larger role in membrane fusion than is commonly assumed in textbook models.
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- Biochemistry and Chemical Biology
- Cell Biology
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