Core genes can have higher recombination rates than accessory genes within global microbial populations
- New York University, United States
Abstract
Recombination is essential to microbial evolution, and is involved in the spread of antibiotic resistance, antigenic variation, and adaptation to the host niche. However, assessing the impact of homologous recombination on accessory genes which are only present in a subset of strains of a given species remains challenging due to their complex phylogenetic relationships. Quantifying homologous recombination for accessory genes (which are important for niche-specific adaptations) in comparison to core genes (which are present in all strains and have essential functions) is critical to understanding how selection acts on variation to shape species diversity and genome structures of bacteria. Here, we apply a computationally efficient, non-phylogenetic approach to measure homologous recombination rates in the core and accessory genome using >100,000 whole genome sequences from Streptococcus pneumoniae and several additional species. By analyzing diverse sets of sequence clusters, we show that core genes often have higher recombination rates than accessory genes, and for some bacterial species the associated effect sizes for these differences are pronounced. In a subset of species, we find that gene frequency and homologous recombination rate are positively correlated. For S. pneumoniae and several additional species, we find that while the recombination rate is higher for the core genome, the mutational divergence is lower, indicating that divergence-based homologous recombination barriers could contribute to differences in recombination rates between the core and accessory genome. Homologous recombination may therefore play a key role in increasing the efficiency of selection in the most conserved parts of the genome.
Data availability
Lists of SRA accession numbers corresponding to the raw reads used to build the multi-sequence alignments analyzed in this manuscript are included as Figure 2 - source data 1 and Figure 3 - source data 1. All SRA files, reference genomes, and complete genome assemblies are available through NCBI. All sequence collections used are listed in Supplementary File 5. For the PubMLST sequence collections, PubMLST was used to identify whole genome sequences (by filtering for strains in the 'Genome Collection' of each species where the sequence length is at least that of the reference genome), then the raw reads were downloaded from NCBI using their SRA numbers. Accession numbers for reference genomes used for each microbial species are also listed in Supplementary File 5.All original code has been deposited at GitHub and is publicly available. Links are given below:- https://github.com/kussell-lab/mcorr- https://github.com/kussell-lab/mcorr-clustering- https://github.com/kussell-lab/ReferenceAlignmentGenerator- https://github.com/kussell-lab/PangenomeAlignmentGenerator
Article and author information
Author details
Funding
National Institutes of Health (R01-GM097356)
- Edo Kussell
Simons Foundation (Simons Foundation Awardee of the Life Sciences Research Foundation)
- Asher Preska Steinberg
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Paul B Rainey, Max Planck Institute for Evolutionary Biology, Germany
Version history
- Preprint posted: September 14, 2021 (view preprint)
- Received: March 10, 2022
- Accepted: June 30, 2022
- Accepted Manuscript published: July 8, 2022 (version 1)
- Version of Record published: September 5, 2022 (version 2)
Copyright
© 2022, Preska Steinberg et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Core genes can have higher recombination rates than accessory genes within global microbial populations
eLife 11:e78533.
https://doi.org/10.7554/eLife.78533
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