Ubiquitination drives COPI priming and Golgi SNARE localization
- Vanderbilt University, United States
- University of Massachusetts Amherst, United States
Abstract
Deciphering mechanisms controlling SNARE localization within the Golgi complex is crucial to understanding protein trafficking patterns within the secretory pathway. SNAREs are also thought to prime COPI assembly to ensure incorporation of these essential cargoes into vesicles, but the regulation of these events is poorly understood. Here we report roles for ubiquitin recognition by COPI in SNARE trafficking and in stabilizing interactions between Arf, COPI, and Golgi SNAREs in Saccharomyces cerevisiae. The ability of COPI to bind ubiquitin, but not the dilysine motif, through its N-terminal WD repeat domain of β'COP or through an unrelated ubiquitin-binding domain (UBD) is essential for the proper localization of Golgi SNAREs Bet1 and Gos1. We find that COPI, the ArfGAP Glo3 and multiple Golgi SNAREs are ubiquitinated. Notably, the binding of Arf and COPI to Gos1 is markedly enhanced by ubiquitination of these components. Glo3 is proposed to prime COPI-SNARE interactions; however, Glo3 is not enriched in the ubiquitin-stabilized SNARE-Arf-COPI complex but is instead enriched with COPI complexes that lack SNAREs. These results support a new model for how posttranslational modifications drive COPI priming events crucial for Golgi SNARE localization.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting file; Source Data files have been provided for all figures.
Article and author information
Author details
Funding
National Institutes of Health (R35GM144123-01)
- Todd R Graham
National Institutes of Health (1R35GM119525)
- Lauren P Jackson
National Institutes of Health (R35GM144112)
- Jason A MacGurn
Pew Charitable Trusts
- Lauren P Jackson
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Felix Campelo, Institute of Photonic Sciences, Spain
Version history
- Preprint posted: November 29, 2021 (view preprint)
- Received: June 9, 2022
- Accepted: July 1, 2022
- Accepted Manuscript published: July 29, 2022 (version 1)
- Version of Record published: August 12, 2022 (version 2)
Copyright
© 2022, Date et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Ubiquitination drives COPI priming and Golgi SNARE localization
eLife 11:e80911.
https://doi.org/10.7554/eLife.80911
