Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin
- Chinese Academy of Sciences, China
Abstract
Microtubules (MTs) and actin filaments (F-actin) function cooperatively to regulate plant cell morphogenesis. However, the mechanisms underlying the crosstalk between these two cytoskeletal systems, particularly in cell shape control, remain largely unknown. Here, we show that introduction of the MyTH4-FERM tandem into KCBP (Kinesin-like Calmodulin-Binding Protein) during evolution conferred novel functions. The MyTH4 domain and the FERM domain in the N-terminal tail of KCBP physically bind to MTs and F-actin, respectively. During trichome morphogenesis, KCBP distributes in a specific cortical gradient, and concentrates at the branching sites and the apexes of elongating branches, which lack MTs but have cortical F-actin. Further live-cell imaging and genetic analyses revealed that KCBP acts as a hub integrating MTs and actin filaments to assemble the required cytoskeletal configuration for the unique, polarized diffuse growth pattern during trichome cell morphogenesis. Our findings provide significant insights into the mechanisms underlying cytoskeletal regulation of cell shape determination.
Article and author information
Author details
Reviewing Editor
- Sheila McCormick, University of California-Berkeley & USDA Agricultural Research Service, United States
Version history
- Received: June 11, 2015
- Accepted: August 18, 2015
- Accepted Manuscript published: August 19, 2015 (version 1)
- Version of Record published: September 18, 2015 (version 2)
Copyright
© 2015, Tian et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin
eLife 4:e09351.
https://doi.org/10.7554/eLife.09351
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