Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA
- St. Jude Children's Research Hospital, United States
- University of Louisville, United States
- The Scripps Research Institute, United States
- Oak Ridge National Laboratory, United States
Abstract
The nucleolus is a membrane-less organelle formed through liquid-liquid phase separation of its components from the surrounding nucleoplasm. Here, we show that nucleophosmin (NPM1) integrates within the nucleolus via a multi-modal mechanism involving multivalent interactions with proteins containing arginine-rich linear motifs (R-motifs) and rRNA. Importantly, these R-motifs are found in canonical nucleolar localization signals. Based on a novel combination of approaches, including smFRET, NMR, and SANS, we propose a model for the molecular organization within liquid-like droplets formed by the N-terminal domain of NPM1 and R-motif peptides, thus providing insights into the structural organization of the nucleolus. We identify multivalency of acidic tracts and folded nucleic acid binding domains, mediated by N-terminal domain oligomerization, as structural features required for phase separation of NPM1 with other nucleolar components in vitro and for localization within mammalian nucleoli. We propose that one mechanism of nucleolar localization involves phase separation of proteins within the nucleolus.
Article and author information
Author details
Reviewing Editor
- Michael K Rosen, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, United States
Version history
- Received: December 6, 2015
- Accepted: January 21, 2016
- Accepted Manuscript published: February 2, 2016 (version 1)
- Accepted Manuscript updated: February 4, 2016 (version 2)
- Version of Record published: February 29, 2016 (version 3)
Copyright
© 2016, Mitrea et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA
eLife 5:e13571.
https://doi.org/10.7554/eLife.13571
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